Cytotoxicity assessment of beta-lapachone in endothelial cells

The selective activity of an antineoplastic drug is related to its ability promote cytotoxic action on tumor cells and preserve the integrity non-neoplastic cells. Beta-lapachone extracted from sawdust Ipe wood, a thick bark tree wood found in Brazilian Cerrado biome. This study aimed evaluate beta-lapachone endothelial cell line. EA.hy926 were seeded two groups, G1 G2, cultured exposed at concentrations 0.0, 0.01, 0.03, 0.1, 0.3, 1 3 μM for 24 hours. remained under normal cultivation conditions G2 was subjected oxidative stress through ischemia reperfusion assay, deoxygenated sealed chamber. cytotoxicity assay performed using tetrazolium reduction method. In G1, ranged 0.0 10.0%; between 6.3%. No statistically significant difference observed obtained values. Moreover, we no cells, results point out that might have preserved cell’s against this experiment. promising result suggests possibility as chemotherapy with activity.